Como melhorar os resultados da Fertilização In Vitro com auxílio do “Assisted Hatching”


Novas tecnologias surgem diariamente com intuito de facilitar, auxiliar e principalmente, melhorar nossa qualidade de vida. 

Da mesma forma, os avanços nos estudos em embriologia humana e no desenvolvimento embrionário in vitro, permitiram nos levar ao ponto em que, hoje, podemos criopreservar óvulos e embriões, selecionar os mais competentes geneticamente e determinar quais apresentam os melhores parâmetros antes da transferência para o útero materno. 

Entretanto, assim como muitas tecnologias nos criam infinitas possibilidades e novos desafios, neste caso, a criopreservação de óvulos e embriões humanos criou a necessidade de se romper uma fina, porém resistente, membrana glicoproteica (açucares + proteínas) que envolve os óvulos e embriões, chamada de zona pelúcida (ZP) . A ZP é a principal barreira para prevenir a polispermia (fertilização do óvulo por mais de um espermatozoide) no processo natural de fertilização. 

Ela é formada por um arcabouço de proteínas  conhecidas como ZP1, ZP2 e ZP3. Este arranjo de glicoproteínas é capaz de sofrer alterações moleculares as quais são induzidas pelo óvulo logo após o primeiro espermatozoide romper a ZP, impedindo que os demais que venham a seguir consigam penetrar esta barreira.

Neste contexto, qual a função do assisted hatching? 

Pois bem, lembrando que algumas tecnologias nos apresentam efeitos indesejados, a criopreservação de óvulos e embriões, causa modificações na Zona Pelúcida, dificultando a saída do embrião já desenvolvido, momentos antes da implantação no útero materno. 

Nestes casos, utilizamos a técnica de assited hatching, que nada mais é que a perfuração controlada da ZP antes da transferência embrionária para o útero materno. 

Esta técnica pode ser realizada pelo embriologista treinado, utilizando uma solução ácida, uma agulha de vidro microscópica ou um sistema a laser, como mecanismo para perfurar a ZP. 

E quais as vantagens  desta técnica?  

Muitas, mas principalmente, ela irá facilitar a saída do embrião da ZP enrigecida pelo processo de criopreservação.

Permitindo que o embrião entre em contato direto com o epitélio da cavidade uterina materna, aderindo ao mesmo e completando o processo de implantação, essencial para o estabelecimento da gestação. 

Por fim, o assited hatching, pode sim, melhorar os resultados nos ciclos de fertilização in vitro em que há criopreservação de óvulos e embriões, sendo de uso rotineiro nos principais centros de reprodução humana, inclusive na Clínica Fertilizar.     

Escrito por:

Dr. Rodrigo Barbano Weingrill

Embriologista da Clínica Fertilizar

Voltar



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